The effect of plant growth-promoting bacteria on wild type, protein phosphatase 2A catalytic subunit mutants of Arabidopsis thaliana and Solanaceae lycopersicum (Gemini tomato)
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This study was designed to gain understanding of the roles of plant growth-promoting traits in tomato cultivars using bacteria with the aim to improve plant health and crop productivity based on microbial inoculation. Rhizosphere bacterial from the hydroponic root of Solanum pennellii tomato plants were isolated, the gene were investigated using PCR, gel electrophoresis and DNA extraction and the bacteria were identified as Sphingobium limneticum and Acidovorax delafieldii using 16S rRNA gene sequencing. The study shows the effect of microbial inoculation on model plants of Arabidopsis thaliana WT-Columbia and Protein phosphatase 2A catalytic subunit mutants (C2, C2C4, C2C5) and tomato plants (Gemini original) using 1/50 Gammborg medium and 1x MS medium (with sucrose or without sucrose) respectively. To investigate the effect of endospheric and rhizosphere strains (WCS714r, Sphingobium limneticum and Acidovorax delafieldii) on WT and mutants, it was discovered that WCS714r and Sphingobium limneticum and Acidovorax delafiedii strains were found to inhibit the primary root length and stimulate the lateral root formation of tomato plants, Arabidopsis WT and the mutants either in vivo or in vitro except some of endospheric and rhizosphere bacteria (Pseudomonas sp., Agrobacterium sp., and Rhizobium sp.,) obtained from Belgium (Abbamondi et al. 2016) which had little effect on plant growth and development. They are represented as 5, 6, 9, 10, 15, 16, and 18. These results suggest that PGPR stimulate plant growth through the inhibition of plant pathogens.
Master's thesis in Biological chemistry